PDF | Cissampelos pareira which is commonly known as velvetleaf belongs to the family Menispermaceae. Numbers of species are available all over the world . Cissampelos pareira f. emarginato-mucronata Chod. & Hassl. Synonym. Cissampelos pareira f. reniformis Chodat & Hassl. Synonym. Cissampelos pareira var. We show that an alcoholic extract prepared from Cissampelos pareira Linn inhibited the replication of dengue viruses in living cells in culture.

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Dengue vaccine efficacy trial: Vascular plant diversity in the sacred grove of Modapalli in Viskhapatnam District of Andhra Pradesh, The effect of Cipa extract on erythrocytes was also assessed, both in ex vivo and in vivo assays, as done for platelets above.

The rest were effective at least against two other DENV serotypes. Infected mice were treated orally with 0. These were procured through local suppliers and identified and authenticated by the resident herbal taxonomist at Ranbaxy Research Laboratories, Gurgaon, India.

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ciasampelos Cissampelos pareira Illustration Credit: DOCX Click here for additional data file. The MST of challenged mice was 19 days under the experimental conditions. In order to meet excellent quality standards, we are supplying Cissampelos Pareira. The initial antiviral screening assay, designated as the type-1 assay, was based on the plaque reduction neutralization test PRNT described earlier for determining DENV-neutralizing antibody titres in sera [ 20 ]. These cells were then treated with Cipa extract and the release of pro-inflammatory cytokines monitored using commercial ELISA kits.

The effect of Cipa extract on platelets was also evaluated in an in vivo experiment using Wistar rats. Pareeira cordifolia Bojer Cissampelos cumingiana Turcz. A type-1 assay was carried out in which DENV-3 was pre-incubated with serial dilutions of a Cipa extract. Further, the observed mean pan-DENV IC 50 values were not significantly different for all four geographic locations pareita.


Flowers are small in size, pedicels filiform.

Strategies for development of dengue virus inhibitors. Views Read Edit View history.

An excellent, if rather terse, guide to the traditional medicinal uses of the plants of the region. Protective efficacy of the recombinant, live-attenuated, CYD tetravalent dengue vaccine in Thai schoolchildren: Organ weights in Cipa extract-treated Wistar rats. As before, cissaampelos and DENV only infections in the absence of Cipa and paracetamol were also set up and analysed in parallel.

Cissampelos bojeriana Miers Cissampelos caapeba L. Technology Biodiversity in India. India has a rich herbal repertoire which is used in traditional ethnomedicine. In assay 3, liquid growth medium was added instead of the methyl cellulose overlay, and followed by analysis of NS1 and virus released into the culture supernatant. Parts of this work have been the subject of patent applications, several of which have been granted [ 3536 ]. Male flowers clustered in the axil of a small leaf; sepals are 4 in number.

Cissampelos Pareira

The plant is very common in hedges at the foothills and up to c. Cissampelos Pareira – Family Name: Handa for his advice and guidance.

I agree to cisssampelos terms and privacy policy. To choose plants likely to provide useful leads, the study utilized knowledge from traditional Indian medicine, Ayurveda, a specific branch of Indian ethnomedicine [ 19 ]. As DF is normally treated with paracetamol, it would be important to ascertain the nature of any interaction between Cipa and this drug. A converse experiment, again in type-1 format, was carried out to determine the inhibitory efficacy of the cissampeloz against DENV-3 stocks cissampleos titers varied over 2 logs.

S3 Fig Total and differential leukocyte counts in Cipa -treated Wistar rat blood. For this purpose a whole cell-based bioassay-guided screening protocol was developed. Cissampelos orinocensis Kunth Cissampelos pannosa Turcz.

Importantly, the reduction in viral titers was sustained over a period of several days. The resultant methanol-free Cipa paste was thoroughly re-suspended in 0.


In the experiment, the results of which are summarized in Table 4Cipa extracts were prepared from plants obtained during the flowering season from four different locations, and evaluated cissapmelos the type-1 assay described above.

Each peak is identified by molecular mass lower number and retention time upper number. This observation has led drug developers to hypothesize that lowering viremia by 1—2 logs may be associated with a favourable prognosis [ 14 ].

S3 Table Biochemical parameters in Cipa extract-treated Wistar rat sera. Effect of Cipa extract on platelets. Groups of 5 adult Wistar rats were orally administered 4 ml 0. It also showed antipyretic and anti-inflammatory effects. The survival data are present in Fig 4. Discussion In the absence of a licensed vaccine or antiviral drug, dengue continues to be a significant global public health problem [ 34 ].

Cissampelos pareira L. | Species | India Biodiversity Portal

Finally, another factor in favour of the second option is that, Cipa plant is available in several parts of the country and extracts prepared using plants from different geographical locations are ciwsampelos similar in terms of their gross overall composition. Recently, indigenous diagnostic tests for the early detection of dengue have become available in India [ 30 ].

At the end of the experiment, the survival data was used to plot Kaplan Meier survival curves and analysed by the log rank test Mantel-Cox test for statistical significance using GraphPad Prism 5 software.

This screening assay revealed 8 of 19 extracts to possess definite anti-DENV-3 inhibitory activity, while one manifested borderline inhibition. Cell viability was assessed using a commercial MTT 3-[4,5-dimethylthiazolyl]-2,5-diphenyltetrazolium bromide assay kit Sigma, cat. It includes published material or suggestions cixsampelos the author or others.