Introducción a la HPLC: aplicación y práctica. Front Cover. Oscar Alberto Quattrocchi. Artes Gráficas Farro, – pages. 6 oct. We are always happy to assist you. Document Description. Introduccion a La HPLC Quattrocchi. Document Share. Documents Related. Results: the HPLC method described for the simultaneous determination of sulphadoxine and pyrimethamine in mL .. Quattrocchi OA, De Andrizzi SA, Laba RF. Introducción a la HPLC Aplicación y. Práctica. Buenos Aires: Artes Gráficas.

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The accuracy and precision did not exceed 4. Moreover, three independent blank human serum samples were checked for any interfering peaks.

Precision and accuracy Quality control samples spiked at three concentrations 0. Unknowns were applied as bands 3 times and references were applied as bands 2 times, each, and distribution of bands was done accordingly the data pair method. Accordingly, a method for the determination of sulfamethazine was developed using differential pulse voltammetry, at pH 11 and with an accumulation time of 3 min.

The results are presented in Tables 1 and 2.

The method was sensitive enough to detect a quantity as low as 0. Wed Aug 11, 4: The developed modified electrode was characterized using cyclic voltammetry quattrocchhi scanning electron microscopy. Calibration curves Calibration curves were constructed using peak-area ratio for the nifedipine and the internal standard carbamazepine as a function of the concentration added. Chailapakul, Talanta 79, Acta8 Acta6 The solutions were protected from light with an aluminum foil wrapping and usually freshly prepared solutions were used.

Care37 In total there are 6 users online:: The chromatographic conditions were arrived at after investigating several parameters, such as detection wavelength, ideal mobile phase and their proportions, internal standards, and optimal conditions for sample extraction.


Introduccion a La HPLC Quattrocchi – Documents

This mixture was vortex-mixed for 1 min and introducicon centrifuged for 10 min at rpm. Precision was calculated by RSD and accuracy was measured from percentage recoveries obtained by the analysis of the quality control samples, determined by linear regression equation of peak area vs. High performance thin layer chromatographic determination of nifedipine in human serum after liquid-liquid extraction.

Actuators B Chromatography Chromatography was carried out on HPTLC glass backed plates 10 x 10 cm and 20 x 10 cm precoated with silica gel Flayer thickness 0.

Dong, Talanta 76, But, I will read this book too, thanks. All the procedure was accomplished under safety conditions and with the needed precautions because the stability of nifedipine.

Serum spiked with nifedipine standard to obtain concentrations of 0. Detection and quantification limits.

Some wavelengths were tried, and nm was selected as working wavelength. Each experiment included the sample extraction step.

Introducción a la HPLC: aplicación y práctica – Oscar Alberto Quattrocchi – Google Books

How to cite this article. There is a list of some recommended books on our web site: Quatttrocchi, Talanta Detection and quantification limits Serum spiked with nifedipine standard to obtain concentrations of 0. This range includes serum concentration found in literature 0. Chile, Pharmaceutics, Selectivity The selectivity bplc the method was examined by preparing and analyzing checked by analyzing human serum samples spiked with nifedipine and the internal standard carbamazepine.

Guzman-Vazquez de Prada, A. No other method using HPTLC was found in the literature, for the quantification of nifedipine in human serum neither in human plasma.


Introduccion a La HPLC Quattrocchi

Therefore, the aim of this study was to develop and validate a fast, sensitive, accurate and precise method using HPTLC for quantitative determination of quattroccih in human serum, suitable for medication adherence studies.

The mobile phase consisted of chloroform: Mon May 16, 8: These values were experimentally verified. Chromatographic chambers were previously saturated with solvent mixture for 20 min.

Nifedipine also may be used in the management of hypertension in patients with low-renin hypertension as black patientscoexisting angina, peripheral vascular-disease, or isolated systolic hypertension, and in geriatric patients.

Separation Science ao free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Acta59 Densitometry readings were carried jntroduccion using the Camag system described before, and with a deuterium quqttrocchi as the radiation source. Wed Mar 07, 6: All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License.

Iowa State University Press, Liquid-liquid extraction yields a good recovery of nifedipine from serum. I’m sorry for my english, I’m Brazilian and I’m learning the language yet. Sulfamethazine; carbon paste; ionic liquid; modified electrodes; voltammetry. Drug-free serum used for the validation of the method was obtained from healthy volunteers. Application of the method.